IGNOU MBC-03 Bioanalytical Techniques - IGNOU Solved Assignment (Latest)

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IGNOU MBC-03 (January 2025 – December 2025) Assignment Questions

1. a) Define the following:

i) pH

ii) Molarity

iii) Numerical aperture

iv) Chemical fixation

v) Rotor

b) Explain the UV-Vis spectrophotometer with suitable diagram.

2. a) What is sub-cellular fractionation? Discuss its process.

b) Write the working principle and basic components of Confocal microscopy.

3. a) Discuss basic theory and application of Atomic force Microscopy.

b) Explain Raman spectroscopy and its applications.

4. a) Define Good Laboratory safety rules. Enlist the chemical safety instructions?

b) Write a brief short note on physical methods of cell disruption.

5. (a) How would you prepare the following laboratory solutions?

i) 250 ml of 0.2 M NaOH

ii) 50 ml of 0.5 N of HCl

b) Explain Bragg’s Law of Diffraction with well labeled diagram.

6. Differentiate between:

a) Paper chromatography and thin layer chromatography

b) FISH and GISH techniques

7. a) Describe isoelectric focusing technique.

b) Discuss the western blotting technique.

8. Describe the basic theory, components and applications of the Gas chromatography.

9. a) Describe the Autoradiography

10. Explain the principle and applications of the following:

a) High-Performance Thin-Layer Chromatography

b) Liquid Chromatography-Mass Spectrometry

IGNOU MBC-03 (January 2024 – December 2024) Assignment Questions

PART-(A)

1. a) Define the following:

i) Buffers
ii) Parts per million (ppm)
iii) Resolving power
iv) Cryofixation
v) Sedimentation coefficient
b) Explain the principle of Beer-Lambert law.

2.a) Discuss the density gradient centrifugation.
b) Explain the working principle and basic components of florescence microscopy.

3.a) Discuss Scanning Tunneling Microscope.
b) Compare ORD and CD Spectroscopy

4. a) Write a brief account note on Laboratory safety rules.
b) Explain the following:
i) Homogenisation
ii) Freeze thaw

5. (a) How would you make the solutions of following concentration?
i) 100 ml of 0.5 M Sucrose solution
ii) 50 ml of 1 Normal solution of HCl.
b) Discuss different methods of ionization in mass spectrophotometry,

PART- (B)

6. Differentiate between:

a) Affinity chromatography and Ion exchange chromatography
b) Northern and Southern technique

7. a) What are supproting media used in electrophoresis?.
b) Discuss the genomic in situ hybridization (GISH) technique.

8. a) State are the major parts of the HPLC Liquid chromatography systems.
b) Describe SDS-PAGE and its significance in characterization of proteins.

9. (a) Define radioisotopes half-life. Write applications of radioactivity.
b) Explain scintillation counting with suitable diagram.

10. Elaborate the principle and applications of the following:

a) LC/MS
b) UPLC

MBC-03 Assignments Details

University	:	IGNOU (Indira Gandhi National Open University)
Title	:	Bioanalytical Techniques
Language(s)	:	English
Code	:	MBC-03
Degree	:	MSCBCH
Subject	:	Biochemistry
Course	:	Core Courses (CC)
Author	:	Gullybaba.com Panel
Publisher	:	Gullybaba Publishing House Pvt. Ltd.