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PLEASE MATCH YOUR ASSIGNMENT QUESTIONS ACCORDING TO YOUR SESSION
IGNOU MBC-04 (January 2025 – December 2025) Assignment Questions
PART-(A)
1. a) Distinguish between :
i) Enzyme Activity and Specific Enzyme Activity
ii) Isomerases and Transferases
b) Explain the effect of pH on enzyme activity.
2. Discuss different types of enzyme assays.
3. a) What are metal activated enzymes?
b) Explain strain and distortion mechanism of catalysis
4. Derive and draw the following:
i) Hanes-Woolf Plot
ii) Eadie-Hofstee Plot
5. Derive Michaelis Menten equation for a mixed inhibitor.
PART- (B)
6. What are allosteric enzymes? Distinguish between MWC and KNF model
7. a) Describe the methods based on polarity of enzyme purification .
b) Write short note on Taq Polymerase.
8. Give an overview of Fatty Acyl multienzyme complex.
9 Describe application of enzymes in the following:
a) Starch industry
b) Wine industry
10. a) Explain the properties of Immobilized enzymes.
b) Describe the rational protein design of enzyme engineering
IGNOU MBC-04 (January 2024 – December 2024) Assignment Questions
PART-(A)
1. Write short notes on the following:
a) Activation energy
b) Active site of enzyme
c) Enzyme activity and its units
d) Metal activated enzymes
2. Distinguish between the following:
i) Isomerases and Ligases
ii) Direct versus Coupled assays
3. What is direct linear plot? Describe its advantages and disadvantages.
4. a) Differentiate between ordered and random sequential reactions.
b) Explain the following:
i) Irreversible enzyme inhbition
ii) Significance of Km and Vmax
5. Derive Michaelis-Menten equation for a competitive inhibitor.
PART- (B)
6. Enumerate the role of multienzyme complex as regulatory enzymes.
7. What are Allosteric enzymes? Discuss the concept of Co-operativity and the Hill equation.
8. Discuss physiological role of Isozymes and their clinical importance.
9. (a) Describe the physical methods of cell disruption.
(b) Explain cross linking method of enzyme immobilization.
10. Discuss the application of enzymes in starch and detergent industry.
