Name: Enzymes and their applications
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IGNOU MBC-04 (January 2026 – December 2026) Assignment Questions

PART-(A)

1. How does an enzyme assay work? Explain its different types.

2. Distinguish between the following:

i) Continuous flow and Stopped flow formats in enzyme kinetics

ii) Sequential and Non-Sequential Substrate enzyme reactions

3. Derive Michaelis Menten equation for a non-competitive inhibitor.

4. Explain the following:

i) Transition State Theory

ii) Strain and Distortion theory of enzyme catalysis

5. Explain the IUPAC system of enzyme classification.

PART- (B)

6. Describe the concept of enzyme engineering.

7. What are proteolytic enzymes? Give a brief account of some of them.

8. Discuss the origin of Isozymes and their nomenclature.

9. (a) Describe the role of multienzyme complexes as regulatory enzymes.

(b) Explain application of enzyme in fruit juice and wine industry.

10. Discuss the methods of enzyme purification based on size or mass.

IGNOU MBC-04 (January 2025 – December 2025) Assignment Questions

PART-(A)

1. a) Distinguish between :
i) Enzyme Activity and Specific Enzyme Activity
ii) Isomerases and Transferases
b) Explain the effect of pH on enzyme activity.

2. Discuss different types of enzyme assays.

3. a) What are metal activated enzymes?
b) Explain strain and distortion mechanism of catalysis

4. Derive and draw the following:
i) Hanes-Woolf Plot
ii) Eadie-Hofstee Plot

5. Derive Michaelis Menten equation for a mixed inhibitor.

PART- (B)

6. What are allosteric enzymes? Distinguish between MWC and KNF model

7. a) Describe the methods based on polarity of enzyme purification .
b) Write short note on Taq Polymerase.

8. Give an overview of Fatty Acyl multienzyme complex.

9 Describe application of enzymes in the following:
a) Starch industry
b) Wine industry

10. a) Explain the properties of Immobilized enzymes.
b) Describe the rational protein design of enzyme engineering

MBC-04 Assignments Details

University	:	IGNOU (Indira Gandhi National Open University)
Title	:	Enzymes and their applications
Language(s)	:	English
Code	:	MBC-04
Degree	:	MSCBCH
Subject	:	Biochemistry
Course	:	Core Courses (CC)
Author	:	Gullybaba.com Panel
Publisher	:	Gullybaba Publishing House Pvt. Ltd.

IGNOU MBC-04 (Jan 26 - Dec 26, Jan 25 - Dec 25) - Solved Assignment

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