Name: Recombinant DNA Technology and its applications
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IGNOU MBC-06 (January 2026 – December 2026) Assignment Questions

PART-(A)

1 Describe restriction endonucleases (REs) and their applications.
2 Illustrate the construction and development of plasmid vector.
3 Write a note on (i) phagemid and (ii) Agrobacterium tumefaciens.

4 (a) How are transformants selected by antibiotic resistance?
(b) Describe RFLP as technique for studying PCR products.

5 Discuss the trp and tet operon.

PART-(B)

1 (a) Differentiate between (i) oligonucleotide and gene probes.
(b) How cDNA library differs from genomic library? Explain.

2 Write a note on the following:
(a)Sanger’s method of DNA sequencing.
(b) PCR based mutagenesis

3 Describe DNA fingerprinting.
4 Explain the applications of plant genetic engineering in agriculture.
5 Write a description on biosafety levels.

IGNOU MBC-06 (July 2024 – December 2025) Assignment Questions

PART-(A)

1. Give an account of modification enzymes used for manipulating DNA in rDNA technology.

2. (a) What are the characteristic features of plasmids as cloning vector?
(b) Explain any two vectors used for cloning large DNA fragments.

3. Describe TMV as a plant viral vector.

4. (a) What is α-complementation?
(b) Describe digital PCR and list its applications.

5. Explain the Lac and Trp operon promoter and its application in rDNA technology.

PART-(B)

1 Describe the applications of oligonucleotide probes.

2. Write a note on the following:
(a) cDNA libraries.
(b) Differential mRNA display.

3. Describe Sanger’s di-deoxy method of DNA sequencing.

4. (a) What are recombinant vaccines? Explain

5. (a) Write a note on herbicide resistant plants.
(b) What are the ethical and biosafety concerns while doing rDNA experiments?

MBC-06 Assignments Details

University	:	IGNOU (Indira Gandhi National Open University)
Title	:	Recombinant DNA Technology and its applications
Language(s)	:	English
Code	:	MBC-06
Degree	:	MSCBCH
Subject	:	Biochemistry
Course	:	Core Courses (CC)
Author	:	Gullybaba.com Panel
Publisher	:	Gullybaba Publishing House Pvt. Ltd.

IGNOU MBC-06 (Jan 26 - Dec 26, Jul 24 - Dec 25) - Solved Assignment

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